Computational analysis of maltose binding protein translocation
نویسندگان
چکیده
منابع مشابه
Role of denaturation in maltose binding protein translocation dynamics.
We present a computational study on the driven transport of the Maltose Binding Protein (MBP) across nanochannels in the framework of coarse-grained modeling. The work is motivated by recent experiments on voltage-driven transport of MBP across nanopores exploring the influence of denaturation on translocation pathways. Our simplified approach allows a statistical mechanical interpretation of t...
متن کاملEngineered Derivatives of Maltose-Binding Protein
Maltose-binding protein (MBP), a member of the periplasmic binding protein family of Gram negative bacteria, is a versatile substrate for protein engineering. In common with other periplasmic proteins, it is extremely protease resistant, and it can fold properly in both the cytoplasmic and periplasmic compartments. It binds a variety of glucose-14-glucose polysaccharides, from maltose and lon...
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Protein translocation in Escherichia coli is mediated by the translocase that, in its minimal form, comprises a protein-conducting pore (SecYEG) and a motor protein (SecA). The SecYEG complex forms a narrow channel in the membrane that allows passage of secretory proteins (preproteins) in an unfolded state only. It has been suggested that the SecA requirement for translocation depends on the fo...
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1. Introduction A major impediment to the production of recombinant proteins in Escheri-chia coli is their tendency to accumulate in the form of insoluble and biologically inactive aggregates known as inclusion bodies. Although it is sometimes possible to convert aggregated material into native, biologically-active protein , this is a time consuming, labor-intensive, costly, and uncertain under...
متن کاملMaltose transport in Aerornonas hydrophila: purification, biochemical characterization and partial protein sequence analysis of a periplasmic maltose-binding protein
Universitat Osna brack, Fach bereich BiologieKhemie, Abtei I ung Mikrobiologie, D-49069 A clinical isolate of Aeromonas hydrophila was demonstrated to transport [14C]maltose with similar kinetics to enteric bacteria (K,,,: 0.3 pM; VM: 22 nmol min-l per lo9 cells). The uptake of [14C]maltose was completely inhibited in the OsnabrUck,-FRG presence of unlabelled maltose or maltodextrins, whereas o...
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ژورنال
عنوان ژورنال: Philosophical Magazine
سال: 2011
ISSN: 1478-6435,1478-6443
DOI: 10.1080/14786435.2011.557670